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Development of competitive ELISA of serum cortisol
Instructions:
Develop competitive ELISA of serum cortisol
Discuss the result of 1. Precision experiment,2. Linearity3. Analytical sensitivity (email)
What does it means of results of each topic and referencing the rule to interpret results?
Enzyme-linked immunosorbent assay (ELISA) kits are widely used to quantify corticosterone levels for the assessment of stress in laboratory animals. The aim of this experiment was simply to evaluate if four different and widely used commercial ELISA assays would yield the same or similar values of corticosterone in serum samples taken from laboratory rats after the mild stress of being held for sampling blood from the saphenous vein. Trunk blood was sampled from 32 male Wistar rats 30 minutes after this mild stress exposure and analysed with each of four commercial ELISA kits.
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